AMPLEX(R) RED REAGENT
[10-Acetyl-3,7-dihydroxyphenoxazine]
CAS: 119171-73-2
Molecular Formula: C14H11NO4
AMPLEX(R) RED REAGENT - Names and Identifiers
AMPLEX(R) RED REAGENT - Physico-chemical Properties
| Molecular Formula | C14H11NO4 |
| Molar Mass | 257.241 |
| Density | 1.459±0.06 g/cm3(Predicted) |
| Melting Point | >250 °C (decomp) |
| Boling Point | 618.6±55.0 °C(Predicted) |
| pKa | 9.41±0.20(Predicted) |
| Storage Condition | 2-8°C |
| Physical and Chemical Properties | Bioactive ADHP is a fluorescent peroxidase substrate (λex = 530 nm, λem = 590 nm). |
| Use | Fluorogenic substrate for peroxidase |
AMPLEX(R) RED REAGENT - Risk and Safety
| Hazard Symbols | Xn - Harmful |
| Risk Codes | 22 - Harmful if swallowed |
| WGK Germany | 3 |
| FLUKA BRAND F CODES | 10-23 |
AMPLEX(R) RED REAGENT - In vitro study
To obtain the parameters K m and k cat for Compound I, two independent methods are used. Initially, the oxidation of ADHP using the injector functionality built-in to the fluorescence plate reader is studied. The auto-injector dispenses the H 2 O 2 to initiate the reaction, as a means of generating a set of progress curves. Analysis for MPO-mediated oxidation of ADHP gives a K m of 31±4 μM and the k cat of 186± 6 s −1. The k obs also increases over the experimental range of ADHP concentrations from 1 to 80 μM and for the converse experiment holding substrate constant over 3 to 45 nM MPO. The apparent second order rate constant obtain from the slope of k obs against ADHP concentration K app on is 2.1±0.2 mM/s.
Last Update：2024-04-10 22:29:15
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